This procedure usually takes approximately 20 – 30 minutes and is scheduled on the same day as the egg retrieval. The local anaesthetic is administered on both sides by a fine needle into the top part of the scrotum. This numbs the whole testes although occasionally some pressure sensation may be experienced.

After the local anaesthetic has taken effect a fine needle is then inserted through the scrotal skin into the epididymis just above the testicle. Under low suction some fluid is aspirated. This fluid is then examined under the microscope for any motile sperm. In about 50% of cases we will find sperm in the fluid, if not then we will need to proceed to a TESA.

This procedure is considered if PESA is unsuitable or unsuccessful. The overall technique of a TESA is similar to a PESA.

With this procedure we insert a small needle directly into the testicle. Under low suction we are able to obtain small amounts of the sperm producing tubules. This tissue then needs to be carefully processed in the laboratory to obtain sperm.